Primer
Platforms Check
Gene specific primer sets for DNA integrity and platforms PCR efficiency check - For L858R/+ , Exon19del/+ , T790M/+ - For G12 or G13 (mutation +) - For V600E/+
Gene specific primer sets for DNA integrity and platforms PCR efficiency check - For L858R/+ , Exon19del/+ , T790M/+ - For G12 or G13 (mutation +) - For V600E/+
*Follow the Master Molecular Pathology Checklist - College of American Pathologists:
CAP MOL.32425 Nucleic Acid Extraction/Isolation/Purification-Phase II
CAP MOL.32430 Nucleic Acid Quantity-Phase II
CAP MOL.32435 Nucleic Acid Quality-Phase II
CAP MOL.34229 Controls - Qualitative Assays-Phase II
CAP MOL.34311 Tolerance Limits - Controls-Phase II
CAP MOL.34352 QC Confirmation of Acceptability-Phase II
CAP MOL.34393 QC Corrective Action-Phase II
CAP MOL.34434 QC Handling-Phase II
CAP MOL.34516 Qualitative Cut-Off Phase II
CAP MOL.30785 Validation Summary Phase II
CAP MOL.31015 Validation Studies - Specimen Types Phase II
CAP MOL.31255 Reference Range Phase II
CAP MOL.31360 Analytical Sensitivity/Lower Limit of Detection Phase II
CAP MOL.31375 Analytical Specificity/Interfering Substances Phase I
CAP MOL.32425 Nucleic Acid Extraction/Isolation/Purification-Phase II
CAP MOL.32430 Nucleic Acid Quantity-Phase II
CAP MOL.32435 Nucleic Acid Quality-Phase II
CAP MOL.34229 Controls - Qualitative Assays-Phase II
CAP MOL.34311 Tolerance Limits - Controls-Phase II
CAP MOL.34352 QC Confirmation of Acceptability-Phase II
CAP MOL.34393 QC Corrective Action-Phase II
CAP MOL.34434 QC Handling-Phase II
CAP MOL.34516 Qualitative Cut-Off Phase II
CAP MOL.30785 Validation Summary Phase II
CAP MOL.31015 Validation Studies - Specimen Types Phase II
CAP MOL.31255 Reference Range Phase II
CAP MOL.31360 Analytical Sensitivity/Lower Limit of Detection Phase II
CAP MOL.31375 Analytical Specificity/Interfering Substances Phase I