Primer

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​​Platforms Check​
Gene specific primer sets for DNA integrity and platforms PCR efficiency check 

• EGFR SQC Primer (Q01D01)

           - For L858R/+ , Exon19del/+ , T790M/+

• KRAS SQC Primer (Q01D02)

           - For G12 or G13 (mutation +)

• BRAF SQC Primer (Q01D03)

           - For V600E/+

​​​FFPE Sample Quality Control Primer
Organ/target specific primer sets for Sample pre-PCR processing check

​​Mutation Enrichment Primer
Organ/target specific primer sets for low allele frequency DNA PCR amplification and detection
ME Primer -> PCR -> Sanger sequence-> analysis​

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*Follow the Master Molecular Pathology Checklist - College of American Pathologists:
CAP MOL.32425 Nucleic Acid Extraction/Isolation/Purification-Phase II
CAP MOL.32430 Nucleic Acid Quantity-Phase II
CAP MOL.32435 Nucleic Acid Quality-Phase II
CAP MOL.34229 Controls - Qualitative Assays-Phase II
CAP MOL.34311 Tolerance Limits - Controls-Phase II
CAP MOL.34352 QC Confirmation of Acceptability-Phase II
CAP MOL.34393 QC Corrective Action-Phase II
CAP MOL.34434 QC Handling-Phase II
CAP MOL.34516 Qualitative Cut-Off Phase II ​
CAP MOL.30785 Validation Summary Phase II
CAP MOL.31015 Validation Studies - Specimen Types Phase II
CAP MOL.31255 Reference Range Phase II
CAP MOL.31360 Analytical Sensitivity/Lower Limit of Detection Phase II
CAP MOL.31375 Analytical Specificity/Interfering Substances Phase I​