TRINITY - A NEW WAY FOR DNA/RNA/Protein PURIFICATION
Silica-based membrane spin column is a widely-used method since it was first published in 1979. The principle behind this method is utilizing a force generated from a centrifuge or a vacuum pump to separate the waste and eluate from the membrane. An automated device designed for this method, however, is usually large and complex and is unfriendly for users. Here we introduce a novel technology - Trinity - to make the automated system more friendly for users.
iCARTRIDGE - LINEARIZE THE WORKFLOW
iCartridge is a special container with all necessary reagents prefilled in the ten same-shaped tubes, and the trinity column put on the other side. Then the iCartridge is sealed automatically and waiting for transferring to the iColumn purification system. During the purification process, Trinity column set “moves” along each reagent well of iCartridge in an orderly manner. Nucleic acid is washed when the Trinity reaches each well, and waste is discarded back into the iCartridge.
iColumn is a fully walk-away system from Lyse, Bind, Wash, Dry to Elute steps. By controlling the pressure difference, the liquid flows up and down through the silica-based membrane featuring non-directional during the purification process, and finally goes to the the elution tube as eluate we need. Robotic arm can connect Trinity by itself and move along with the iCartridge. All movements are in same axis.