AccuPure Plant RNA Mini Kit(R30096)
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Rice leaf total RNA purification
| Method | AccuPure Plant RNA Mini Kit | |||||
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| Sample | Rice leaf | |||||
| Sample name | TN67 | 1-1-1 | 1-1-2 | 1-1-4 | 1-1-6 | 1-1-12 |
| Sample volume (mg) | 50 | |||||
| 260/280 | 2.13 | 2.16 | 2.10 | 2.13 | 2.13 | 2.13 |
| 260/230 | 1.97 | 1.70 | 2.06 | 1.79 | 1.20 | 2.09 |
| Yield(μg) | 10.1 | 6.4 | 12.4 | 13.4 | 12.2 | 13.2 |
 Total RNA of Rice leaf samples (50mg fresh weight) were purified by iColumn. Followed by reverse-transcription to cDNA and performed the PCR reaction. Left part are the amplify results of housekeeping gene - Actin. Right part are the amplify results of target gene. Both sizes are correct and well amplified. |
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