In order to break the cell membrane of the sample to release nucleic acid, enzyme and Lysis buffer are added to the sample to achieve Lysis
As for binding, a buffer solution and ethanol are then added to the sample, and then the binding solution is passed through the silicon membrane with positive pressure. Finally, the nucleic acid will bind to the silicon membrane and stay on the silicon membrane
In order to obtain the purified nucleic acid, we use the ethanol-based wash buffer to wash out the cell debris, protein and other salt contamination from the silica membrane.
The elution buffer added to the silica membrane removes the nucleic acid from the membrane.Finally, we get the highly purified nucleic acid.